James McGrath - Current Research
Size Based Filtration of Oligodeoxynucleotides
With this unique SimPore filtration system, we are testing the hypothesis that for each deoxyribose damage, created by direct ionization, there will be a corresponding free base release.
The unique characteristics of SimPore filters make it possible to address key problems addressing the mechanism by which ionizing radiation damages DNA via the direct effect. One is to use the filters to purify oligodeoxynucleotides. For example, a synthetic preparation of a 16 mer will be contaminated with shorter sequences: 15 mer, 14 mer, â¦.1 mer. With these filters, the quantity of small fragments can be significantly reduced while retaining most of the desired larger oligodeoxynucleotide. Another application is to determine the ratio between strand break formation and the release of free base.