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Contact Info

James L. McGrath, Ph.D. Department of Biomedical Engineering University of Rochester work Box 270168 Rochester, NY 14627-0168 office: Goergen Hall 306 p 585-273-5489 f 585-273-4746 McGrath

James McGrath - Current Research

Size Based Filtration of Oligodeoxynucleotides

With this unique SimPore filtration system, we are testing the hypothesis that for each deoxyribose damage, created by direct ionization, there will be a corresponding free base release.

The unique characteristics of SimPore filters make it possible to address key problems addressing the mechanism by which ionizing radiation damages DNA via the direct effect. One is to use the filters to purify oligodeoxynucleotides. For example, a synthetic preparation of a 16 mer will be contaminated with shorter sequences: 15 mer, 14 mer, ….1 mer. With these filters, the quantity of small fragments can be significantly reduced while retaining most of the desired larger oligodeoxynucleotide. Another application is to determine the ratio between strand break formation and the release of free base.