Dynamic response of neutrophils to chemokine stimulus
In the vasculature, inflammation leads not only to the expression of new adhesive ligands on the endothelial cell surface, but also the expression of signaling molecules (chemokines) that attract and activate leukocytes. An important chemokine that acts on neutrophils is IL-8, which is typically found immobilized on sugar structures (the gylcocalyx) on the endothelium. In order to imitate the natural presentation of chemokines to neutrophils, we immobilize IL-8 on a fractalkine stalk on the surface of a bead. By this approach, we can control the timing of the exposure of the cells to stimulus very precisely. We have completed several projects in this area, and continue to pursue others.