Raman and angular-scattering microscopy

Raman scattering reports on the chemical content of a target, while angularly-resolved (elastic) scattering gives information about the structural content. We have developed an optical system that combines the two methods on a microscopy platform. Ongoing studies include (a) fundamental comparisons to various scattering theories and (b) applications to the monitoring of immune cells as they respond to stimuli. The image shows a comparison of an angular-scattering pattern from a 5-micron diameter bead, measured experimentally and then modeled using a focused-beam electromagnetic scattering theory called Generalized Lorenz-Mie Theory.