BME Seminar: Chen-Yuan Dong

Developing Intravital Multiphoton Microscopy for Biomedical Research

Department of Physics National Taiwan University

Abstract

Traditionally, the difficulty of accessing internal organs has often limited the study of complex cellular physiology to in vitro, monolayer systems. While in vitro studies using techniques such as optical imaging and electron microscopy have provided a wealth of dynamic and structural information of cells and tissues, the critical components of biophysical phenomena in complex three-dimensional tissues are missing. Therefore, much of our efforts have been focused at developing microscopy techniques leading to the study of physiological phenomena in vivo. In this presentation, I will discuss the efforts we have made in developing intravital multiphoton microscopy (IMM) for investigating biophysical and physiological events in living mouse animal models. In the process, we found that multiphoton microscopy can be useful for medical diagnostic purposes, quantification of tissue thermodynamic properties, evaluation of tissue engineering products, and the elucidation of hepatic metabolism in vivo. Future developments and impacts of our studies for biomedical engineering will be discussed.

(Coffee will be served)