BME Seminar Series: Flavius C. Pascut
Chemical and Biological Applications of Raman Spectroscopy
Since its discovery by Raman and Krishnan in 1926, the Raman effect has enjoyed an explosion of new applications. The past 20 years in the Raman spectroscopy history are the most exciting with the demonstration of the first Raman application on live cells. Raman spectroscopy has several particularly useful features: non-invasive, non-destructive, not requiring labels, high spatial resolution, no sample preparation and having the ability to investigate biological samples in their physiological conditions. Perhaps the most attractive feature of Raman spectroscopy lies in its ability to detect spectroscopic information at a molecular level without the use of chemical labels or cell fixation. Such high specificity enables the identification and characterisation of individual biochemical molecules inside complex biological samples such as cells and tissues. Several examples of the applicability of Raman spectroscopy, from nanoscale, micro to macro scale, will be demonstrated. They include: molecular orientation of self-assembling peptide nanotubes, programmed cell death (apoptosis), cell based biosensors, imaging immunological synapses, label free phenotype identification, stem cells differentiation, parasite infection and automated imaging and diagnosis of basal cell carcinoma during Mohs surgery.